Try a CAS stain. = PAS with Chromic acid Perhaps this fungus needs something stronger than Periodic acid.
Gudrun -----Ursprüngliche Nachricht----- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Weaver, Colin Gesendet: Mittwoch, 05. November 2008 18:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fungal staining Hi - everyone - does anyone know of any fungi that can not be demonstrated by PAS stain? We appear to have one in a cow placenta which is obvious on Grocott and H&E but do not stain on PAS. The micro lab isolated Absidia spp but the hyphae in the Grocott are septate but I believe Absidia are aseptate. Any ideas would be most welcome. Colin Weaver VLA Thirsk England Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet