CD31 for sure. I do recall most surface markers I used tended to be unstable.
Jackie O' "Gudrun Lang" <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 11/20/2008 09:17 AM Please respond to [EMAIL PROTECTED] To <histonet@lists.utsouthwestern.edu> cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria -----Ursprüngliche Nachricht----- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 [EMAIL PROTECTED] www.ihctech.net www.ihcrg.org -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
