Hi All: I have been working up the PMS2 and MLH2 mismatched repair genes and I am not satisfied with my results. I am using the Cell Marque antibodies and the Ventana XT using CC1, mild, standard and I have tried the antibodies with block and amplification, and antibody times from 32 min- 1 hour and the stain is not satisfactory. Anyone who has these antibodies in their lab and would like to share your protocols, I would be grateful for your help. The MSH2 and MSH6 are working great.
Lena Spencer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet