You could also try to add some ammonium hydroxide in tap water after the Mayers and before the tap water rinse. This helps us in the bluing on our mayers H&E frozen tissue stain line. Add approx 5ml to 150ml of water from 30 sec to 1 min you will have to play with it a little . This blues our tissues up nicely.
Matt Lunetta HT Longmont United Hospital Message: 1 Date: Sat, 10 Jan 2009 13:33:09 -0500 From: "Weems, Joyce" <jwe...@sjha.org><~!B*+R^&>Subject: RE: [Histonet] H&E help To: <lpw...@sbcglobal.net>, "Charles, Roger" <rchar...@state.pa.us>,<~!B*+R^&> "Histonet" <histonet@lists.utsouthwestern.edu><~!B*+R^&>Message-ID:<~!B*+R^&> <5d64396a0d4a5346bebc759022aaeaa5257...@itsssxm01v6.one.ads.che.org><~!B*+R^&>Content-Type: text/plain; charset="us-ascii" Also, warm water works best for bluing, if you have that option.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, January 10, 2009 11:00 AM To: 'Charles, Roger'; 'Histonet' Subject: RE: [Histonet] H&E help It might not be a hematoxylin problem. It might be too much eosin. Try one of the following: - cut the eosin time down to 1 minute, and if it's still too pink/not blue enough, cut the eosin time down to 30 seconds. - and/or, change the first 95% alcohol after the eosin to 70% alcohol. That will help to put out more eosin than 95%. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] H&E help Hello All, TGIF!!!! I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the H&E staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is "are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 13 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet