Bluing in tap water is quite often done but has the disadvantage that your protocol cannot be repeated in other Lab because not all tap waters have the same composition of "bluing quality". As any other chemical reaction it is better to blue in warm tap water (it will take less time) but you can also blue in cold water (at the temperature the water comes out of the tap). As to the time that will be determined by the "blue intensity" you are looking for and it will be less time in summer than in winter. All these intrinsic inconsistencies is what has determined the wide acceptance of the practice of bluing in a chemically prepared solution (like lithium carbonate or weak ammonia water). René J.
--- On Mon, 1/12/09, Eva Permaul <e...@georgetown.edu> wrote: From: Eva Permaul <e...@georgetown.edu> Subject: [Histonet] bluing in tap water? To: histonet@lists.utsouthwestern.edu Date: Monday, January 12, 2009, 9:35 AM Good morning, I was wondering if someone uses tap water to blue their slides after Hematoxyline. If yes, do you use warm or cold water and for how long? Thanks, Eva Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
