The Wright Cell Imaging Facility at University Health Network in Toronto has compiled information on reducing autofluorescence from the Histonet archives into a booklet. Very useful.
http://www.uhnres.utoronto.ca/facilities/wcif/fdownload2.html Bob On Thu, Jan 15, 2009 at 1:36 PM, Patten, Nicole (NIH/NIAAA) [F] < patte...@mail.nih.gov> wrote: > Hi- > > I am having a horrible time with autofluorescence in my human brain FFPE > tissue. I have been using Sudan Black which helps a little, but the > background is still pretty bad. The tissue is usually cut at 10-15um. > > Does anyone have any other suggestions? Has anyone tried photobleaching > the tissue prior to staining with any success? I attempted this once > with no luck... > > Any help would be very much appreciated! Thanks in advance. > > Nicole > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet