I was taught 4 changes of 50% EtOH, 1-1.5 hours each. The goal is to get
most of the picrates out of the tissue for long-term storage (of blocks)
I don't know if one can ever get all of the yellow color out.
Geoff
Jennifer Anderson wrote:
Hello.
I am looking for information regarding procedures for tissue handling
after Bouin's fixation, prior to processing in a standard automated
tissue processor (VIP). We are handling pig, mouse, rat, and human
samples, and will fix in Bouin's for 48-72 hrs. I've been told to rinse
until all yellow is washed out, and people generally rinse in 70%
alcohol or water. Sometimes it take days to wash all of the picric acid
out.
Thank you so much for your wealth of information!
Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11404 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
jander...@halozyme.com
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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