In the UK (where I used to run a clinical lab) we also would turn off
the cryostat in the morning, wait for it to defrost then just before
going home for the evening heat up some formalin in the microwave and
place that in the chamber and leave overnight.
It certainly kills everything, but it is also kinda toxic to your
lungs! I wouldn't suggest it but it is a 'severe cleaning' option,
especially if you have had something nasty in there.
This was about 5 years ago, the rules may have changed, and Yes, I do
realise just how bad that is for you and everyone around!
Caroline
Caroline Miller
Co-Manager
J David Gladstone Institutes Histology and Microscopy Core
1650 Owens St
San Francisco
CA 94158
http://www.gladstone.ucsf.edu/gladstone/site/histology/
cmil...@gladstone.ucsf.edu
On Feb 7, 2009, at 1:11 PM, Lee & Peggy Wenk wrote:
95-100% also work, for bacteria, fungus, viruses. They will not work
on
killing spores. That's where the 70% is to be used, as the water
(30% part)
is needed to soften the spore wall, so that the alcohol (70% part)
can get
inside.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Friday, February 06, 2009 6:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Disinfecting Cryostat
Why no 95%? We have been using it in perpituity with no bad effects.
The
alcohol has evaporated from the inside surfaces by morning.
Claire
________________________________
From: histonet-boun...@lists.utsouthwestern.edu on behalf of gayle
callis
Sent: Fri 2/6/2009 11:11 AM
To: 'Louise Hartson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Disinfecting Cryostat
We use 70% ethanol on gauze (just damp) to wipe down inside of
cryostat. Do
not use 95% or 100% alcohol, as 70% is the most effective for
disinfection,
commonly used in biohoods. We pick up little trimmings with first
dampened
gauze (or kim wipe) then re-wipe with a second gauze. We prefer
gauze for
its flexibility. Also, if you put a kimwipe or gauze behind knife
holder to
l catch trimmings then you can fold up kimwipe, and lift "garbage"
out,
intact, and go to biohazard container before wipe down. We try to
avoid too
much alcohol on knife holder parts since alcohol ruins lubricants
needed for
good operation. Check with your cryostat manufacturer to see what
they
recommend. It pays to wipe down under and over sliding glass door to
counterattack biohazardous "cling-ons" found on your gloves, and where
people have touched the instrument. 70% alcohol will not kill prions.
Water based disinfectants freeze in cryostat. Eventually you need
scheduled
disinfection with cryostat in defrosted mode with approved
disinfecting
agent.
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