For general information:
Brazilliant is the product name for Anatech's red nuclear stain
solution. It is available and will continue to be available for the
foreseeable future. The dye brazilin used to prepare Brazilliant also
will continue to be available from Anatech.
Ada T. Feldman
ANATECH LTD.
1020 Harts Lake Road
Battle Creek, MI 49037
Phone: 800.262.8324
Fax: 269.964.8084
email: adafeld...@anatechltdusa.com
website: www.anatechltdusa.com
On Feb 26, 2009, at 11:42 PM, Robert Richmond wrote:
Sarah Jones at Dako responds - and Sarah, please forgive me for
quoting you onto Histonet, but this seems to me to be a public
message:
Brazilliant will soon not be available for some time. The
government in the
state of Pernambuco in Brazil has declared a 10 year moratorium on
the
felling of the Pernambuco trees from which this dye is made. This
took
place in September of 2007, if I remember correctly. Violin bows
are also
made from this tree. I was doing some work here at Dako trying to
use it in
place of Nuclear Fast Red. But when I learned of this moratorium
(at the
Biological Stain Commission Annual Meeting in June of 2008), I
stopped
working on it. I believe I sent you some of my photomicrographs
taken at
that time. I never heard back from you, so I thought you lost
interest. I
can send them to you again if you want me to. Just let me know.
And Allen Smith responded:
I have used brazalum, made by substituting brazilin for
hematoxylin in the recipe for Mayer's hemalum. It stains nuclei a
slightly deeper red than nuclear fast red. Colorfastness is
excellent. I have 40-year old slides stained with brazalum that
still look as they did on the day I made them.
Allen A. Smith, Ph.D.
Barry University School of Podiatric Medicine
**********************
I think this clarifies the problem. I would hope that something could
be worked out with the Brazilian government for the very small amount
of brazil wood histologists require, but the Brazilian government has
been difficult about issues like this - see a number of citations in
Science over the last few years.
Sarah, I was much impressed by your photomicrographs of stains with
brazalum nuclear counterstaining, and I apologize for having failed to
reply earlier.
Bob Richmond
Samurai Pathologist
Knoxville TN
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