Valentin,
Diluting acetone in no different than diluting another solvent e.g. absolute ethanol. I generally measure the water into a graduated cylinder, then add the solvent. So if you want 50% acetone, measure out 50 ml distilled water, then add acetone to the 100 ml mark on a 100 ml capacity graduated cylinder. I don't know of any reason why you could not do a different percentage of acetone, whether higher or lower either. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
