Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice.
2009-03-06 TF 发件人: Colleen Forster 发送时间: 2009-03-06 00:57:29 收件人: tifei 抄送: iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题: Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: > hi, CD31 works great > in my section alpha-SMA also works > > another way is to perfuse the brain with BSA-rhodamine. > you will get the fluorescence without the need of staining. > > > 2009-03-05 > > > > TF > > > > 发件人: iskaliora > 发送时间: 2009-03-05 18:49:11 > 收件人: histonet > 抄送: ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ > 主题: [Histonet] staining brain vessels > > I was wondering if anybody might have an idea with the following > problem we are experiencing: we want to stain for blood vessels in > sections of mouse brain. Our experimental tissues have been fixed > overnight in 4% paraformaldehyde and have been sitting in PBS since. > We have tried staining with antibodies against desmin, SMA, and > collagen but we get NO specific signal. We recently tried a non-fixed > mouse brain and got desmin to work immediately. The problem is that > we need to use the fixed brains because they are our experimental > model and it would take too long (2 years to be exact) to generate the > same samples. If anybody has come across such a problem before, or > has a specific protocol for vessels that works on PFA fixed brain, we > would appreciate the suggestions! > thanks in advance! > Irini > ----------------------------------------------------- > Irini Skaliora, PhD > Investigator C᾽ (Assistant Professor) > Developmental Biology Division > Biomedical Research Foundation of the Academy of Athens (BRFAA) > Soranou Efessiou 4 > Athens 11527 > tel. +30-210-6597203 (office) > tel. +30-210-6597482 (lab) > fax. +30-210-6597545 > email: iskali...@bioacademy.gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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