Dear Fellow Histonetters,

There are 3 counterstains that can be used with the Sanderson's Rapid Bone 
Stain (RBS).  We primarily used the acidified acid fuchsin, however, we had 
clients that only used a van Gieson counterstain.  Light green can also be 
used.  We normally stained for 1 minute ground sections in the RBS, rinsed in 
water and then counterstained.  As Gayle mentioned, intensity of the RBS or the 
counterstain can be increased with addtional time. 

Cathy Mayton

Wasatch Histo Consultants, Inc. 
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