Hello Histonetters, I have processed some strips of cartilage (about 2cm long, 3mm of height, 2mm wide) with my standard protocol.
I cannot section them. They just start to break away as soon as they hit the blade. I tried to section them really cold and also at RT, but that did not have any effect. I guess sth went wrong when processing (other kinds of tissue were fine). My protocol: Reagent Time [h] Spin (Microm ST120) NBF 1 1 70% Isopropanol 1 1 80% Isopropanol 1 1 95% Isopropanol 1 1 abs Isopropanol 1 1 abs Isopropanol 1 1 abs Isopropanol 1 1 Xylene 1 1 Xylene 1 1 Xylene 1 1 Paraffin I 1 1 Paraffin II 1 1 Maybe someone can help me out and give some hints. Thanks a lot! V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
