I would not use liquid nitrogen. The safest way to do this is to put the blocks in a refrigerator freezer for a couple of hours. Remove quickly, remove caps, wrap in paper towels and hit with a hammer. Be sure to wear protective clothing, eye covers and gloves. When the glass has broken into the towel put the towel in a sharps container, rinse the block and procees with the sectioning.
Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, April 03, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could "use liquid nitrogen", but had no specifics. Any help will be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
