Good morning: I have good luck by simply using haematoxylin and eosin staining....the eosinophils pop right out by virtue of their bright pink/red granules! When used in conjunction with nuclear morphology, one can, with confidence, call these cells eosinophils.
I have never performed IHC for eosinophils, but just did a quick search and found this helpful website. I am sure there are more websites like this, but this one will certainly get you started. http://www.antibodybeyond.com/reviews/cell-markers/eosinophil-marker.htm Best of luck -->mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: prit...@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost) Sent: Tuesday, April 07, 2009 10:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosinophilic granulocytes Hi Is anyone of you aware of a marker/method to stain eosinophilic granulocytes in mouse lung tissue which is formalin fixed and paraffin embedded? Thanks Joost Bruijntjes TNO Quality of Life Zeist Holland TNO.NL <http://www.tno.nl/> Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijnt...@tno.nl <mailto:joost.bruijnt...@tno.nl> Disclaimer <http://www.tno.nl/tno/email/> This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet