Hi All, I am having variable staining when performing IHC. I seem to get an edge effect where the edge of the tissue shows increased DAB staining compared to the middle of the tissue. I have tried testing many fixation variables and still haven't found an edge affect culprit. I'm wondering if anyone in the community has experienced and corrected this problem. Thanks for you help!
-Sam Perry Dana Farber Cancer Institute Boston MA Research Technician The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet