Don't forget the effects on decalcified Bone Marrow Cores. Even if you use EDTA decalcification solution, there will be diminished FE staining on Bone Marrow Cores or any other tissue that is decalcified and then stained for FE. Regards,Akemi
Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 E-Mail: akemiat3...@yahoo.com --- On Thu, 4/9/09, Rene J Buesa <rjbu...@yahoo.com> wrote: From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu, "kyle ayres" <ayresk...@yahoo.com> Date: Thursday, April 9, 2009, 1:53 PM Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse effect is greater and because of that, when possible, the decalficication should be done with EDTA. René J. --- On Thu, 4/9/09, kyle ayres <ayresk...@yahoo.com> wrote: From: kyle ayres <ayresk...@yahoo.com> Subject: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 3:31 PM Are there negative effects associated with acid decalcification and special stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE. Thanks, Kyle Ayres HT BS Nacogdoches Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet