I have a colleague who requires snap frozen rodent brains to perform Mass Spec on 10micron frozen sections mounted on plates. Because OCT interferes with mass spec, they are currently using ethanol to slow the freezing process to prevent cracking of the brain in liquid nitrogen. The alcohol, however, causes some detriment to the surface of the brains - in science-speak "they get mushy". Does anyone have any suggestions for snap freezing brains without any substrate while preventing cracking?
Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
