Hi all i am working on brain. After perfusion with 4% PFA, I put the brain in the fixative for post-fixation overnight. Then I put the brain into 30% surcose until sink. Then I perform cryostat or microtome sections.
Do you think it is necessary to perform antigen retrieval of DCX (doublecortin), ki67, and caspase-3? I got very weak staining signals. I am not sure whether it is due to the low primary incubation concentration? Or insufficient antigen binding site exposure? 2009-04-27 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
