We never tired that but I think you should not do that. For the sake of the 2nd batch of sections; also, the solution pH, concentration, ion strength after boiling should have changed/
2009-05-01 TF 发件人： Jason McGough 发送时间： 2009-04-30 23:15:31 收件人： ih...@googlegroups.com; histonet@lists.utsouthwestern.edu 抄送： 主题： [Histonet] Reusing Citrate Antigen Retrieval Buffer We are looking into using our .01M Citrate Antigen Retrieval Buffer in our Dako PT Link for Antigen Retrieval on our IHC stains. The question that we are wondering about is anybody reusing this solution for multiple times? If so, how many times? What is your dilution? Like the High pH Antigen Retrieval solution from Dako, it is recommended to use up to 3 time before replacement. We are having several problems with the High pH antigen retrieval solution from Dako and want to try Citrate, since that is what we have been using for many years with great results. We previously used the Pascal Pressure Cooker from Dako for Antigen Retrieval but now have the PT Link. Thank you in advance for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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