1) Acid alcohol: I use several drops of concentrated HCL in 95% ethanol (1% HCL in 70% alcohol well too). Simply dip your rinsed slides (or sections) into solution a couple of times, rinse and check them... a dip in water with about 10drops of ammonia should follow as well... 2) Saturated picric acid, followed by a couple of dips in water with several drops of ammonia, rinse and check 3) 2% Iron alum or ferric chloride (times vary and can range from a couple of dips to several minutes...)
-Matt Close _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet