> Good luck freezing a brain that is full of alcohol! Have you checked the freezing point of alcohol? > Why are you doing this?
I think that moire often than not, people ask before they think ;-) On Fri, May 15, 2009 at 11:56 AM, Geoff McAuliffe <mcaul...@umdnj.edu>wrote: > Greetings TF: > > Good luck freezing a brain that is full of alcohol! Have you checked the > freezing point of alcohol? > Why are you doing this? > Immersing a whole brain in 70% alcohol, why?? > 30% sucrose is a cryoprotectant so the brain is not full of holes from ice > crystals.Alcohol defeats this purpose. > > Geoff > > > > TF wrote: > >> Hi, i dont want to use PFA for the brain fixation (rat). >> Now I tried to perfuse the rat with saline, followed with 70% alcohol. >> Then I do post-fixation at room temperature for 24 hours. >> I also tried saline perfusion, then I directly put the whole brain into >> 70% alcohol. >> Is this fine? >> >> Also, for dehydration before cutting frozen sections on a >> cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~ >> >> 2009-05-15 >> >> >> TF _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcaul...@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet