Here is one reference that might be of use: Philippe Bertheau "Variability of immunohistochemical reactivity on stored paraffin slides" J Clin Pathol 1998;51:370-374
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, 27 May 2009 4:16 AM To: histonet@lists.utsouthwestern.edu; f...@ufl.edu Subject: [Histonet] IHC control debate Hello, I need help from all of our IHC expert guru's! We have a debate going on about the known positive control blocks. All are FFPE with 8-48 hrs in formalin. All have been tested and have been approved for use as positive controls. We currently cut the positive control from the block as needed. I have learned that some controls do start to deteriorate over time, tonsils for example. But, is there a list, an article, SOMETHING that can guide me so that we can cut some of these controls ahead of time? This would not only save tech time, but would further the use of our controls. In cutting the block, some of the good control is wasted and some positives are hard to find. My argument is that the purchased controls work wonderfully, even after a very long time and our improved detection kits have improved so much over the years, it makes this possible. If there is no published article out there, I implore you experts to please help us in this area. Would also like to hear from the technical experts from our suppliers too! Until then, the debate continues on!!!!!!! Thank you!! Lynette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet