Dear Dzung, I wonder whether the perfusion would be good in an euthanized animal with the circulation arrested. It would be better to try it under deep anesthesia. Certainly perfusion would be better
Regards Raghul RCC laboratories India Pvt. Ltd Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion? Thanks much, Dzung NIAID ------------------------------ Message: 5 Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT) From: Phyllis Thaxton <dch...@yahoo.com> Subject: [Histonet] Cardboard Paraffin Catchers To: Histonet@lists.utsouthwestern.edu Message-ID: <372922.65853...@web43506.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Does anyone remember the little cardboard microtome trays for paraffin waste? If so please email me the company that makes them. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ------------------------------ Message: 6 Date: Mon, 29 Jun 2009 12:07:04 -0400 From: Merced M Leiker <lei...@buffalo.edu> Subject: Re: [Histonet] Signs of good perfusion To: "Thach, Dzung (NIH/NIAID) [E]" <thac...@niaid.nih.gov>, histonet@lists.utsouthwestern.edu Message-ID: <1d8e958050abad54bd852...@cdywxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Live going pale is a good sign, your tissues of interest going pale is an even better sign, but fluid coming out of the mouth (or even the nose, or additionally, any kind of bloating or swelling in the animal) is a bad sign. You may not be able to get good perfusion (pale tissues) if this happens before your tissues turn pale, as the pressure is too high causing fluid to leak out of the vasculature....ideally you want to push the blood out through the the hole you made in the right atrium, not through the walls of the vessels. at what rate do you perfuse? if this happens a lot slow it down. Hope this helps. --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" <thac...@niaid.nih.gov> wrote: > Hi Everyone!! > > I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am > nicking the upper right atrium of heart to collect the gushed out blood > and then perfusing through ventricle using a 21G butterfly needle and > peristaltic pump. Sometimes I see the lungs swelling up and fluid comes > out of mouth. Occasionally, I see the liver fade to light pink. Most of > the time the paws become white. But the brain and spinal cord (my > tissues of interest) are always white, and seem to have been perfused. I > was wondering how to improve this to get more consistent good perfusions, > and what signs should I look for to indicate good perfusion? > > Thanks much, > > Dzung > NIAID > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 7 Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT) From: Steven Coakley <sjchta...@yahoo.com> Subject: [Histonet] WI/MI To: Histonet@lists.utsouthwestern.edu Message-ID: <385293.93112...@web38204.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I'm looking for a few techs from Northern WI or Northern MI to let me know when HT positions become availiable. Thanks ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 32 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet