but he's not using fixative.
--On Tuesday, June 30, 2009 10:06 AM +0100 "Edwards, R.E."
<r...@leicester.ac.uk> wrote:
A stiff tail!.
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thach,
Dzung (NIH/NIAID) [E] Sent: 29 June 2009 16:31
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Signs of good perfusion
Hi Everyone!!
I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am
nicking the upper right atrium of heart to collect the gushed out blood
and then perfusing through ventricle using a 21G butterfly needle and
peristaltic pump. Sometimes I see the lungs swelling up and fluid comes
out of mouth. Occasionally, I see the liver fade to light pink. Most of
the time the paws become white. But the brain and spinal cord (my
tissues of interest) are always white, and seem to have been perfused. I
was wondering how to improve this to get more consistent good perfusions,
and what signs should I look for to indicate good perfusion?
Thanks much,
Dzung
NIAID
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_______________________________________________
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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