Steve You need to keep you reagent buckets filled to the middle line. The 100% alcohol should be to the maximum line especially after the eosin. You have some running of solution down the top of the slide causing uneven staining.
When we hand stain we move the baskets vigorously up and down. That will take care of any run off of solution. Automation is a wonderful thing but it can only work as well as it is set up to operate. Hence the suggestion to fill some buckets with less solution and the 100% with maximum amount. 100% will clear the top of the slides so solution will not run down the slide or so you won't have uneven staining at the top of the slide. Hope this makes sense. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com "Asquith Stephen" <stephen.asquith@ petermac.org> To Sent by: <histonet@lists.utsouthwestern.edu> histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Leica Autostainer XL Problems 06/29/2009 08:24 PM Hi All, We have a Leica Autostainer XL that we do all our routine HE slides on. The section on the upper end of the slide is often unevenly stained. We have tried many things to improve the situation including; Ensuring wash tubs are flowing correctly and Increasing dips at each staining tub. If we "use" the same program but do it by hand the problem goes away. Any suggestion that may help would be most welcome. Steve Stephen Asquith Microscopy Imaging and Research Core Facility Peter MacCallum Cancer Centre Locked Bag #1 A'Beckett Street East Melbourne 8006 Email: stephen.asqu...@petermac.org Phone +61-3-9656 1244/1243 Fax +61-3-9656 1411 This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet