Hello... I am attempting to use this antibody, CD168 (2D6), also known as Rhamm or Hmmr.
I tried using 10mM citrate buffer but that did not work at all. I did not see any signal. I also have tried using the 1mMEDTA antigen retrieval solution but the control tissue, normal human testis, was no longer there after AR is done. I have tried other control tissues still no results. It was suggested to me to use Excel slides which I did and the tissue stayed but was very degraded after AR. I am using a 1:50 and 1:100 dilution of this antibody but I see a bit of cytoplasm lighting up at 1:50. The data sheet says that it should show on the cell membrane. But the signal only showed up after I left the antibody overnight at 4 degrees. I have also tried the antibody at room temp for 1 hour to no avail. I am using DAKO products, protien block, Mouse Polymer and DAB+ which have worked in other IHC's recently done. I have tried two different lots of this antibody with no success. Any suggestions? Thanks Joie -- Joie Haviland, HT (ASCP) Senior Research Assistant, Dr. McDonnell's Lab Y5.6057 Department of Hematopathology MD Anderson Cancer Research Center 713-792-0705 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
