I did a von kossa day the other since it was sunny out and never thought to use a hema counterstain. I will try that next time! I use ammonia water too as a bluing agent.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, July 17, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Hi Jan, (Carrie Disbrow) 2. Quality Stuff (kristen arvidson) 3. RE: Quality Stuff (Mike Pence) 4. Re: Quality Stuff (Rene J Buesa) 5. Re: Biological hood with grossing station (Joseph Saby) 6. RE: Hi Jan, (Tony Henwood) 7. Re: Hi Jan, (Victor Tobias) 8. Re: Quality Stuff (theci...@yahoo.com) 9. VonKossa's calcium stain (karine cadoret) 10. RE: VonKossa's calcium stain (Tony Henwood) 11. Re: VonKossa's calcium stain (Jack Ratliff) 12. Protocol for fluorescence of myofibers (J.P.H.M. van den Wijngaard) 13. NSH Meeting in Alabama (mtitf...@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Thu, 16 Jul 2009 16:15:24 -0400 From: "Carrie Disbrow" <dis...@shands.ufl.edu> Subject: [Histonet] Hi Jan, To: <histonet@lists.utsouthwestern.edu> Message-ID: <4a5f521b.72ac.005...@shands.ufl.edu> Content-Type: text/plain; charset=US-ASCII Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie ------------------------------ Message: 2 Date: Thu, 16 Jul 2009 14:14:36 -0700 (PDT) From: kristen arvidson <arvidsonkris...@yahoo.com> Subject: [Histonet] Quality Stuff To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <685857.31127...@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. ------------------------------ Message: 3 Date: Thu, 16 Jul 2009 16:21:00 -0500 From: "Mike Pence" <mpe...@grhs.net> Subject: RE: [Histonet] Quality Stuff To: "kristen arvidson" <arvidsonkris...@yahoo.com>, "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <661949901a768e4f9cc16d8af8f2838c017a3...@is-e2k3.grhs.net> Content-Type: text/plain; charset="iso-8859-1" There is NO margin of error acceptable in mislabeling blocks or slides. I expect 100% compliance with this in my department. When you have like specimens all day like derm, you cannot make labeling errors. Mike -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday, July 16, 2009 4:15 PM To: histonet Subject: [Histonet] Quality Stuff Hello, I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 16 Jul 2009 14:47:23 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Quality Stuff To: histonet <histonet@lists.utsouthwestern.edu>, kristen arvidson <arvidsonkris...@yahoo.com> Message-ID: <720913.73313...@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=utf-8 There are no acceptable "standards" for mistakes. The present tendency of implementing the "6σ method" in the lab is to precisely eliminate mistakes, not to set an "acceptable" limit. René J. --- On Thu, 7/16/09, kristen arvidson <arvidsonkris...@yahoo.com> wrote: From: kristen arvidson <arvidsonkris...@yahoo.com> Subject: [Histonet] Quality Stuff To: "histonet" <histonet@lists.utsouthwestern.edu> Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 16 Jul 2009 14:48:30 -0700 (PDT) From: Joseph Saby <saby_josep...@yahoo.com> Subject: Re: [Histonet] Biological hood with grossing station To: Golden State Acrylic Designs <gsacrylicdesi...@gmail.com>, histonet@lists.utsouthwestern.edu Message-ID: <143120.31950...@web33807.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Although I really like MOPEK, another source for the East Coast would be TBJ. ________________________________ From: Golden State Acrylic Designs <gsacrylicdesi...@gmail.com> To: histonet@lists.utsouthwestern.edu Sent: Thursday, July 16, 2009 9:34:56 AM Subject: [Histonet] Biological hood with grossing station Is the a source for a biological hood with grossing station othe than (Thermo-Fisher) Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 17 Jul 2009 08:42:48 +1000 From: "Tony Henwood" <antho...@chw.edu.au> Subject: RE: [Histonet] Hi Jan, To: "Carrie Disbrow" <dis...@shands.ufl.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <b9eaf61856077f47bf9be2f89afc555202fb0...@hedwig.nch.kids> Content-Type: text/plain; charset="us-ascii" Hi Carrie, The NSH convention in Alabama will also be my first. It has only taken me 30 years to finally get to one. Assuming I don't get lost I hope to see you all there (?turn right at Honolulu, left at Los Angeles, then second exit on the left?) I'll be the short tubby man with the funny accent. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Friday, 17 July 2009 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hi Jan, Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R & D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 7 Date: Thu, 16 Jul 2009 15:49:39 -0700 From: Victor Tobias <vic...@pathology.washington.edu> Subject: Re: [Histonet] Hi Jan, To: Tony Henwood <antho...@chw.edu.au> Cc: Carrie Disbrow <dis...@shands.ufl.edu>, histonet@lists.utsouthwestern.edu Message-ID: <4a5fae83.30...@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Tony, LOL, someone with a funny accent in Alabama. Something only a local can appreciate. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Tony Henwood wrote: > Hi Carrie, > > The NSH convention in Alabama will also be my first. > It has only taken me 30 years to finally get to one. > > Assuming I don't get lost I hope to see you all there (?turn right at > Honolulu, left at Los Angeles, then second exit on the left?) > > I'll be the short tubby man with the funny accent. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie > Disbrow > Sent: Friday, 17 July 2009 6:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hi Jan, > > > Hi Jan, > Thanks for your input! I have two A. S. degrees. One in veterinary > nursing/technology and one in histology. And I have an AA where all my > electives were biology,chemistry and micro. Then I'll have a BS in > veterinary nursing/management. I'm starting a molecular program in > January. So, I have a strong skills in courses you mentioned. I'm sure I > want to do the R & D techniques but not so sure about the management! > The other thing about a histology career is learning how vast the field > is. Did anyone ever have a counselor in their program that explained the > different types of positions? I'm looking forward to attending the NSH > convention in October. It will be my first one! Carrie > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Fri, 17 Jul 2009 00:37:34 +0000 From: theci...@yahoo.com Subject: Re: [Histonet] Quality Stuff To: "Rene J Buesa" <rjbu...@yahoo.com> Cc: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1122935475-1247791003-cardhu_decombobulator_blackberry.rim.net-15317022...@bxe1123.bisx.prod.on.blackberry> Content-Type: text/plain; charset="utf-8" I also run a derm lab where we gross and write cassettes. The doctors medical assistants make mistakes every week so I set up a double checking system where one tech accessions and check numbers and writes slides. Then I gross and make one final qc check. this is only possible since I have low volume - not too sure about specific setups for larger lab qc Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa <rjbu...@yahoo.com> Date: Thu, 16 Jul 2009 14:47:23 To: histonet<histonet@lists.utsouthwestern.edu>; kristen arvidson<arvidsonkris...@yahoo.com> Subject: Re: [Histonet] Quality Stuff There are no acceptable "standards" for mistakes. The present tendency of implementing the "6σ method" in the lab is to precisely eliminate mistakes, not to set an "acceptable" limit. René J. --- On Thu, 7/16/09, kristen arvidson <arvidsonkris...@yahoo.com> wrote: From: kristen arvidson <arvidsonkris...@yahoo.com> Subject: [Histonet] Quality Stuff To: "histonet" <histonet@lists.utsouthwestern.edu> Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 17 Jul 2009 12:34:51 +1000 (EST) From: "karine cadoret" <kcado...@amc.edu.au> Subject: [Histonet] VonKossa's calcium stain To: <histonet@lists.utsouthwestern.edu> Message-ID: <001001ca0687$296b2600$7c4172...@edu.au> Content-Type: text/plain; charset="US-ASCII" Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia ------------------------------ Message: 10 Date: Fri, 17 Jul 2009 13:43:37 +1000 From: "Tony Henwood" <antho...@chw.edu.au> Subject: RE: [Histonet] VonKossa's calcium stain To: "karine cadoret" <kcado...@amc.edu.au>, <histonet@lists.utsouthwestern.edu> Message-ID: <b9eaf61856077f47bf9be2f89afc555202fb0...@hedwig.nch.kids> Content-Type: text/plain; charset="us-ascii" Karine, Either Hx will do, though I would not have used a haematoxylin since it will lake with the calcium forming a blue stained deposit. I would expect it to mask the silver of the von-kossa stain. I would recommend 1% neutral red, ethylene green or even a light eosin counterstain. The silver "stained" calcium deposits should then stand out quite well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of karine cadoret Sent: Friday, 17 July 2009 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VonKossa's calcium stain Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 11 Date: Thu, 16 Jul 2009 23:15:37 -0500 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: Re: [Histonet] VonKossa's calcium stain To: karine cadoret <kcado...@amc.edu.au> Cc: "<histonet@lists.utsouthwestern.edu>" <histonet@lists.utsouthwestern.edu> Message-ID: <blu0-smtp1843c194df1f376fd822d5ae...@phx.gbl> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes What is your tissue of interest? Why not do the Von Kossa stain first and then counterstain with MacNeal's tetrachrome. This way you employ the use of a metachromatic stain for the rest of the tissue instead of just a nuclear staining hematoxylin. Jack On Jul 16, 2009, at 9:34 PM, "karine cadoret" <kcado...@amc.edu.au> wrote: > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing instead > of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Fri, 17 Jul 2009 15:38:38 +0100 From: "J.P.H.M. van den Wijngaard" <j.p.vandenwijnga...@amc.uva.nl> Subject: [Histonet] Protocol for fluorescence of myofibers To: histonet@lists.utsouthwestern.edu Message-ID: <ba9cb7db4f0c4577.4a609...@amc.uva.nl> Content-Type: text/plain; charset=us-ascii Dear experts of histonet, Given my formal background (chemistry synthesis) and current experience (cardiovascular research) my question may seem either too simple of not well directed. Please forgive my inexperience in these matters. In short, I am looking for a simple method that enhances the fluorescence of the cardio myofibers. In our institution, we have constructed a special setup allowing for investigation of vessel morphology. This is carried out by infusing a fluorescent plastic that polymerizes into an organ and then serially slicing the specimen while after each slice a high resolution image is taken of the remaining bulk material. As such, we can create high resolution 3D images of the vasculature, e.g. of a heart or kidney. Recently we have extended our setup (we are using a 16mpixel cooled camera which also allows very long exposure times) and are now trying to visualize the muscle fibers of the heart. For this we use a powerled (around 400nm) and image at around 600nm which seems to generates images that show autofluorescence of either collagen or muscle (I am unsure which this may be). Given these initial promising results, I would like to visualize the muscle fibers in more detail by using a staining protocol that would allow to stain post mortem hearts. I have gathered information so far that includes the use of ALA or Bouin's solution but there may be much better protocols suitable for this problem. I appreciate all feedback, thank you in advance, Jeroen Jeroen PHM van den Wijngaard, PhD Department of Biomedical Engineering and Physics Academic Medical Center Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands Tel: +31 (20) 5668796 ------------------------------ Message: 13 Date: Fri, 17 Jul 2009 11:33:34 -0400 From: mtitf...@aol.com Subject: [Histonet] NSH Meeting in Alabama To: histonet@lists.utsouthwestern.edu Message-ID: <8cbd50b14f6ae2e-ef8-3...@webmail-md01.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings! Michael Titford Pathology USA Mobile AL ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 68, Issue 21 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.
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