Why dont you use NFR as counterstain for Von kassa? > From: histonet-requ...@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 68, Issue 21 > To: histonet@lists.utsouthwestern.edu > Date: Fri, 17 Jul 2009 10:02:55 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Hi Jan, (Carrie Disbrow) > 2. Quality Stuff (kristen arvidson) > 3. RE: Quality Stuff (Mike Pence) > 4. Re: Quality Stuff (Rene J Buesa) > 5. Re: Biological hood with grossing station (Joseph Saby) > 6. RE: Hi Jan, (Tony Henwood) > 7. Re: Hi Jan, (Victor Tobias) > 8. Re: Quality Stuff (theci...@yahoo.com) > 9. VonKossa's calcium stain (karine cadoret) > 10. RE: VonKossa's calcium stain (Tony Henwood) > 11. Re: VonKossa's calcium stain (Jack Ratliff) > 12. Protocol for fluorescence of myofibers > (J.P.H.M. van den Wijngaard) > 13. NSH Meeting in Alabama (mtitf...@aol.com) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 16 Jul 2009 16:15:24 -0400 > From: "Carrie Disbrow" <dis...@shands.ufl.edu> > Subject: [Histonet] Hi Jan, > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <4a5f521b.72ac.005...@shands.ufl.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi Jan, > Thanks for your input! I have two A. S. degrees. One in veterinary > nursing/technology and one in histology. And I have an AA where all my > electives were biology,chemistry and micro. Then I'll have a BS in veterinary > nursing/management. I'm starting a molecular program in January. So, I have a > strong skills in courses you mentioned. I'm sure I want to do the R & D > techniques but not so sure about the management! > The other thing about a histology career is learning how vast the field is. > Did anyone ever have a counselor in their program that explained the > different types of positions? I'm looking forward to attending the NSH > convention in October. It will be my first one! > Carrie > > > > > > ------------------------------ > > Message: 2 > Date: Thu, 16 Jul 2009 14:14:36 -0700 (PDT) > From: kristen arvidson <arvidsonkris...@yahoo.com> > Subject: [Histonet] Quality Stuff > To: histonet <histonet@lists.utsouthwestern.edu> > Message-ID: <685857.31127...@web65709.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello, > I work in a derm lab and we do all the grossing. We hand write on all of our > blocks and slides, so you can imagine we have mislabelings from time-to-time. > I was wondering if other labs have acceptable limits set for errors such as > these, and if so what are they like? I am working on setting standards and > corrective actions for errors in the lab. Thank you for any input. > > > > > ------------------------------ > > Message: 3 > Date: Thu, 16 Jul 2009 16:21:00 -0500 > From: "Mike Pence" <mpe...@grhs.net> > Subject: RE: [Histonet] Quality Stuff > To: "kristen arvidson" <arvidsonkris...@yahoo.com>, "histonet" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <661949901a768e4f9cc16d8af8f2838c017a3...@is-e2k3.grhs.net> > Content-Type: text/plain; charset="iso-8859-1" > > There is NO margin of error acceptable in mislabeling blocks or slides. I > expect 100% compliance with this in my department. When you have like > specimens all day like derm, you cannot make labeling errors. > > Mike > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen > arvidson > Sent: Thursday, July 16, 2009 4:15 PM > To: histonet > Subject: [Histonet] Quality Stuff > > > Hello, > I work in a derm lab and we do all the grossing. We hand write on all of our > blocks and slides, so you can imagine we have mislabelings from time-to-time. > I was wondering if other labs have acceptable limits set for errors such as > these, and if so what are they like? I am working on setting standards and > corrective actions for errors in the lab. Thank you for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 4 > Date: Thu, 16 Jul 2009 14:47:23 -0700 (PDT) > From: Rene J Buesa <rjbu...@yahoo.com> > Subject: Re: [Histonet] Quality Stuff > To: histonet <histonet@lists.utsouthwestern.edu>, kristen arvidson > <arvidsonkris...@yahoo.com> > Message-ID: <720913.73313...@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > There are no acceptable "standards" for mistakes. The present tendency of > implementing the "6σ method" in the lab is to precisely eliminate mistakes, > not to set an "acceptable" limit. > René J. > > --- On Thu, 7/16/09, kristen arvidson <arvidsonkris...@yahoo.com> wrote: > > > From: kristen arvidson <arvidsonkris...@yahoo.com> > Subject: [Histonet] Quality Stuff > To: "histonet" <histonet@lists.utsouthwestern.edu> > Date: Thursday, July 16, 2009, 5:14 PM > > > Hello, > I work in a derm lab and we do all the grossing. We hand write on all of > our blocks and slides, so you can imagine we have mislabelings from > time-to-time. I was wondering if other labs have acceptable limits set for > errors such as these, and if so what are they like? I am working on setting > standards and corrective actions for errors in the lab. Thank you for any > input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 5 > Date: Thu, 16 Jul 2009 14:48:30 -0700 (PDT) > From: Joseph Saby <saby_josep...@yahoo.com> > Subject: Re: [Histonet] Biological hood with grossing station > To: Golden State Acrylic Designs <gsacrylicdesi...@gmail.com>, > histonet@lists.utsouthwestern.edu > Message-ID: <143120.31950...@web33807.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Although I really like MOPEK, another source for the East Coast would be TBJ. > > > > > ________________________________ > From: Golden State Acrylic Designs <gsacrylicdesi...@gmail.com> > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, July 16, 2009 9:34:56 AM > Subject: [Histonet] Biological hood with grossing station > > Is the a source for a biological hood with grossing station othe than > (Thermo-Fisher) > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Fri, 17 Jul 2009 08:42:48 +1000 > From: "Tony Henwood" <antho...@chw.edu.au> > Subject: RE: [Histonet] Hi Jan, > To: "Carrie Disbrow" <dis...@shands.ufl.edu>, > <histonet@lists.utsouthwestern.edu> > Message-ID: <b9eaf61856077f47bf9be2f89afc555202fb0...@hedwig.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Hi Carrie, > > The NSH convention in Alabama will also be my first. > It has only taken me 30 years to finally get to one. > > Assuming I don't get lost I hope to see you all there (?turn right at > Honolulu, left at Los Angeles, then second exit on the left?) > > I'll be the short tubby man with the funny accent. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie > Disbrow > Sent: Friday, 17 July 2009 6:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hi Jan, > > > Hi Jan, > Thanks for your input! I have two A. S. degrees. One in veterinary > nursing/technology and one in histology. And I have an AA where all my > electives were biology,chemistry and micro. Then I'll have a BS in > veterinary nursing/management. I'm starting a molecular program in > January. So, I have a strong skills in courses you mentioned. I'm sure I > want to do the R & D techniques but not so sure about the management! > The other thing about a histology career is learning how vast the field > is. Did anyone ever have a counselor in their program that explained the > different types of positions? I'm looking forward to attending the NSH > convention in October. It will be my first one! Carrie > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 7 > Date: Thu, 16 Jul 2009 15:49:39 -0700 > From: Victor Tobias <vic...@pathology.washington.edu> > Subject: Re: [Histonet] Hi Jan, > To: Tony Henwood <antho...@chw.edu.au> > Cc: Carrie Disbrow <dis...@shands.ufl.edu>, > histonet@lists.utsouthwestern.edu > Message-ID: <4a5fae83.30...@pathology.washington.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Tony, > > LOL, someone with a funny accent in Alabama. Something only a local can > appreciate. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > vic...@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Tony Henwood wrote: > > Hi Carrie, > > > > The NSH convention in Alabama will also be my first. > > It has only taken me 30 years to finally get to one. > > > > Assuming I don't get lost I hope to see you all there (?turn right at > > Honolulu, left at Los Angeles, then second exit on the left?) > > > > I'll be the short tubby man with the funny accent. > > > > Regards > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > > Laboratory Manager & Senior Scientist > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > > > > > > -----Original Message----- > > From: histonet-boun...@lists.utsouthwestern.edu > > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie > > Disbrow > > Sent: Friday, 17 July 2009 6:15 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Hi Jan, > > > > > > Hi Jan, > > Thanks for your input! I have two A. S. degrees. One in veterinary > > nursing/technology and one in histology. And I have an AA where all my > > electives were biology,chemistry and micro. Then I'll have a BS in > > veterinary nursing/management. I'm starting a molecular program in > > January. So, I have a strong skills in courses you mentioned. I'm sure I > > want to do the R & D techniques but not so sure about the management! > > The other thing about a histology career is learning how vast the field > > is. Did anyone ever have a counselor in their program that explained the > > different types of positions? I'm looking forward to attending the NSH > > convention in October. It will be my first one! Carrie > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************* > > This email and any files transmitted with it are confidential and intended > > solely for the use of the individual or entity to whom they are addressed. > > If you are not the intended recipient, please delete it and notify the > > sender. > > > > Views expressed in this message and any attachments are those of the > > individual sender, and are not necessarily the views of The Children's > > Hospital at Westmead > > > > This note also confirms that this email message has been > > virus scanned and although no computer viruses were detected, The Childrens > > Hospital at Westmead accepts no liability for any consequential damage > > resulting from email containing computer viruses. > > ********************************************************************** > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 8 > Date: Fri, 17 Jul 2009 00:37:34 +0000 > From: theci...@yahoo.com > Subject: Re: [Histonet] Quality Stuff > To: "Rene J Buesa" <rjbu...@yahoo.com> > Cc: Histonet <histonet@lists.utsouthwestern.edu> > Message-ID: > <1122935475-1247791003-cardhu_decombobulator_blackberry.rim.net-15317022...@bxe1123.bisx.prod.on.blackberry> > > Content-Type: text/plain; charset="utf-8" > > I also run a derm lab where we gross and write cassettes. The doctors medical > assistants make mistakes every week so I set up a double checking system > where one tech accessions and check numbers and writes slides. Then I gross > and make one final qc check. this is only possible since I have low volume - > not too sure about specific setups for larger lab qc > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Rene J Buesa <rjbu...@yahoo.com> > > Date: Thu, 16 Jul 2009 14:47:23 > To: histonet<histonet@lists.utsouthwestern.edu>; kristen > arvidson<arvidsonkris...@yahoo.com> > Subject: Re: [Histonet] Quality Stuff > > > There are no acceptable "standards" for mistakes. The present tendency of > implementing the "6σ method" in the lab is to precisely eliminate mistakes, > not to set an "acceptable" limit. > René J. > > --- On Thu, 7/16/09, kristen arvidson <arvidsonkris...@yahoo.com> wrote: > > > From: kristen arvidson <arvidsonkris...@yahoo.com> > Subject: [Histonet] Quality Stuff > To: "histonet" <histonet@lists.utsouthwestern.edu> > Date: Thursday, July 16, 2009, 5:14 PM > > > Hello, > I work in a derm lab and we do all the grossing. We hand write on all of > our blocks and slides, so you can imagine we have mislabelings from > time-to-time. I was wondering if other labs have acceptable limits set for > errors such as these, and if so what are they like? I am working on setting > standards and corrective actions for errors in the lab. Thank you for any > input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 9 > Date: Fri, 17 Jul 2009 12:34:51 +1000 (EST) > From: "karine cadoret" <kcado...@amc.edu.au> > Subject: [Histonet] VonKossa's calcium stain > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <001001ca0687$296b2600$7c4172...@edu.au> > Content-Type: text/plain; charset="US-ASCII" > > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing instead of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > > > ------------------------------ > > Message: 10 > Date: Fri, 17 Jul 2009 13:43:37 +1000 > From: "Tony Henwood" <antho...@chw.edu.au> > Subject: RE: [Histonet] VonKossa's calcium stain > To: "karine cadoret" <kcado...@amc.edu.au>, > <histonet@lists.utsouthwestern.edu> > Message-ID: <b9eaf61856077f47bf9be2f89afc555202fb0...@hedwig.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Karine, > > Either Hx will do, though I would not have used a haematoxylin since it > will lake with the calcium forming a blue stained deposit. I would > expect it to mask the silver of the von-kossa stain. > > I would recommend 1% neutral red, ethylene green or even a light eosin > counterstain. > The silver "stained" calcium deposits should then stand out quite well. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of karine > cadoret > Sent: Friday, 17 July 2009 12:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] VonKossa's calcium stain > > > Hi, > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > hematoxylin (which takes 6 months to ripen) ? > > Also, can I simply use homemade scott's tapwater for blueing instead of > using a lithium carbonate solution ? > > > > Thank you for your help, > > > > Karine Cadoret > > Fish health laboratory manager > > National Center for Marine Conservation and Resource Sustainability > > Newnham, TAS > > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 11 > Date: Thu, 16 Jul 2009 23:15:37 -0500 > From: Jack Ratliff <ratliffj...@hotmail.com> > Subject: Re: [Histonet] VonKossa's calcium stain > To: karine cadoret <kcado...@amc.edu.au> > Cc: "<histonet@lists.utsouthwestern.edu>" > <histonet@lists.utsouthwestern.edu> > Message-ID: <blu0-smtp1843c194df1f376fd822d5ae...@phx.gbl> > Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes > > What is your tissue of interest? Why not do the Von Kossa stain first > and then counterstain with MacNeal's tetrachrome. This way you employ > the use of a metachromatic stain for the rest of the tissue instead of > just a nuclear staining hematoxylin. > > Jack > > > On Jul 16, 2009, at 9:34 PM, "karine cadoret" <kcado...@amc.edu.au> > wrote: > > > Hi, > > > > When doing a VonKossa stain in order to demonstrate calcium in tissue, > > does it matter much if I use Mayer's hematoxylin instead of Ehrlich's > > hematoxylin (which takes 6 months to ripen) ? > > > > Also, can I simply use homemade scott's tapwater for blueing instead > > of > > using a lithium carbonate solution ? > > > > > > > > Thank you for your help, > > > > > > > > Karine Cadoret > > > > Fish health laboratory manager > > > > National Center for Marine Conservation and Resource Sustainability > > > > Newnham, TAS > > > > Australia > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 12 > Date: Fri, 17 Jul 2009 15:38:38 +0100 > From: "J.P.H.M. van den Wijngaard" <j.p.vandenwijnga...@amc.uva.nl> > Subject: [Histonet] Protocol for fluorescence of myofibers > To: histonet@lists.utsouthwestern.edu > Message-ID: <ba9cb7db4f0c4577.4a609...@amc.uva.nl> > Content-Type: text/plain; charset=us-ascii > > > > Dear experts of histonet, > > Given my formal background (chemistry synthesis) and current experience > (cardiovascular research) my question may seem either too simple of not well > directed. Please forgive my inexperience in these matters. > > In short, I am looking for a simple method that enhances the fluorescence of > the cardio myofibers. > > In our institution, we have constructed a special setup allowing for > investigation of vessel morphology. This is carried out by infusing a > fluorescent plastic that polymerizes into an organ and then serially slicing > the specimen while after each slice a high resolution image is taken of the > remaining bulk material. As such, we can create high resolution 3D images of > the vasculature, e.g. of a heart or kidney. > > Recently we have extended our setup (we are using a 16mpixel cooled camera > which also allows very long exposure times) and are now trying to visualize > the muscle fibers of the heart. For this we use a powerled (around 400nm) and > image at around 600nm which seems to generates images that show > autofluorescence of either collagen or muscle (I am unsure which this may > be). Given these initial promising results, I would like to visualize the > muscle fibers in more detail by using a staining protocol that would allow to > stain post mortem hearts. I have gathered information so far that includes > the use of ALA or Bouin's solution but there may be much better protocols > suitable for this problem. > I appreciate all feedback, thank you in advance, > > Jeroen > > Jeroen PHM van den Wijngaard, PhD > > Department of Biomedical Engineering and Physics > Academic Medical Center > Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands > Tel: +31 (20) 5668796 > > > > > > > > > ------------------------------ > > Message: 13 > Date: Fri, 17 Jul 2009 11:33:34 -0400 > From: mtitf...@aol.com > Subject: [Histonet] NSH Meeting in Alabama > To: histonet@lists.utsouthwestern.edu > Message-ID: <8cbd50b14f6ae2e-ef8-3...@webmail-md01.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > With the NSH Annual Convention in Alabama this year, I don't want any corny > jokes on the Histonet?about my adopted home state!? Might hurt my feelings! > > Michael Titford > Pathology USA > Mobile AL > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 68, Issue 21 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
