We were having a similar issue with our IHC staining on the Ventana Benchmark Classic. We were able to track the problem down to the slides. We never found out what was wrong with the slides but as soon as we changed lot numbers the staining issue cleared up. We were able to track down the problem with the assistance of our Ventana technical rep. Through her we were able to find out that several institutions across Canada were experiencing similar staining problems. We were all using the same lot number of slides. She also informed me that there was one manufacturer of the plus slides and they were sold under multiple vendors. Good luck with tracking down your problem.
Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, British Columbia Canada -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jacobs, Genie Sent: Thursday, July 16, 2009 5:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] immuno staining We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for our immuno stains. We recently purchased the Ventana Ultra and really like it. We dual mount the control and patient. We have recently had several cases where the control was positive but the patient was not. We repeated the stain and the patient was positive the second time. We have had issues with washing but have increased the time in the oven Has anyone else had this problem We don't have a lot of variables that are different Thanks for any input The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet