You can do it, I have done it, BUT you will have to wash very well the well (no pun intended) to make sure that there are no cross-reactions. René J.
--- On Fri, 7/31/09, Michael Patrick <mpatric...@hotmail.com> wrote: From: Michael Patrick <mpatric...@hotmail.com> Subject: [Histonet] IHC free floating sections To: histonet@lists.utsouthwestern.edu Date: Friday, July 31, 2009, 3:14 AM I would like to stain free floating sections (fixed rat brain; 50um). Is it necessary to transfer sections to "clean" culture wells with a paint brush every time I wash or incubate with antibodies? I find this causes significant damage to my tissue (especially since I do a lot of washes etc.). Could I simply use a pipette to draw out the old solution and then replace with the appropriate solution? What do most people do in these situations? Much thanks Mike _________________________________________________________________ Stay in the loop and chat with friends, right from your inbox! http://go.microsoft.com/?linkid=9671354_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
