Judy: Amos has a good suggestion.
Below is a link to a Movat procedure from Penn, we discontinued our clinical Movat procedure due to lack of use (though it is a great stain). In the attached link, note the comments about the wash and differentiation steps. The Movat is nice in that it allows you to differentiate elastic, fibrin and collagen. Check after the alcian blue to see if the fibrin is already stained blue. Adjust concentrations and timing if necessary. Ensure that the alkaline alcohol is completely removed. Good luck. http://www.med.upenn.edu/mcrc/histology_core/movat.shtml Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Sunday, August 09, 2009 6:03 AM To: jud...@u.washington.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Fibrin stain with Movat's? Hi, Have you tried a Sirius red (in sturated picric acid) with a Weigart's hamatoxylin for the Nucleii? I usually don't bother counterstaining the nucleii with this stain, but I suppose it would work. You wouuld probably want to do the hematoxylin after the Sirius red since it is an acidic solution and would bleach out the hematoxylin otherwise. While this isn't a Movat's I think it might do the trick for you. Amos Message: 2 Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) From: "Judith L. Williams" <jud...@u.washington.edu> Subject: [Histonet] Good Fibrin stain with Movat's? To: histonet@lists.utsouthwestern.edu Message-ID: <pine.lnx.4.43.0908071021110.27...@hymn33.u.washington.edu> Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet