If the G in 4F1G is glutaraldehyde the glut. will probably kill off the
immunoreactivity (depending on the antigen in question).
How long are the embryos being fixed? Remember, formalin works slowly so
5-7 days may be necessary.-
I would try a formalin + alcohol mix or formalin-alcohol-acetic acid.
Also, yolk is hard to fix well so don't use that as a criterion for good
fixation.
Geoff
Cheryl Crowder wrote:
Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.
After processing they are mushy and almost amorphous. Has anyone any
suggestions for a fixative that will give the embryos more form and still
have the tissue capable of doing IHC? I had thought of 4F1G. Any help is
really appreciated.
Cheryl
Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803
225-578-9734
FAX: 225-578-9720
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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