Here is the polychrome formula I use. Jim Polychrome Methylene Blue Stain
Tissue and Fixation Frozen sections and formalin-fixed, paraffin embedded Objective Rapid differential staining of tissue sections. Metachromatic stain. Reagents Stock solutions: A. 12% aqueous potassium carbonate, 100 ml. B. 1% aqueous methylene blue, 4000 ml. C. 10% aqueous glacial acetic acid, 50 ml. Staining solutions: 1. Mix 100 ml of A with 5000 ml of B. 2. Gently boil for 60 minutes. Cool to room temperature. 3. Add 50 ml of C. 4. Store in a loosely capped bottle for further oxidation. Procedure 1. Dip frozen sections in stain for 5 seconds. 2. Rinse excess stain from slide. 3. Mount in water with a cover glass if desired. Results Nuclei blue, cytoplasm, muscle, connective tissue, pink to purple. Note: The sections are not permanent. Solution works best after aging for at least 12 months, recognizable by a rich tinctoral quality. Increase stock solution volumes to make a larger quantity of working stain. Filter before use. Reference: Terry, BT, J. Lab and Clinical Medicine, Vol. 13, page 550, 1928. "Woodward, Denise" <denise.woodw...@uconn.edu> Sent by: histonet-boun...@lists.utsouthwestern.edu 08/11/2009 11:23 AM To "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] whipf's polychrome procedure Hello all, One of our veterinary pathology residents from France is looking for a staining procedure for "Whipf's Polychrome". Anyone have it and willing to share? Thanks, Denise Long Woodward University of Connecticut Connecticut Veterinary Medical Diagnostic Laboratory Dept. of Pathobiology and Veterinary Sciences _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet