Hi Dorothy, I've not used hexane. I think 2-methly butane is more commonly used if you are, as I suspect, snap freezing in a solvent chilled in liquid nitrogen. The solvent just needs to have a low freezing point. 2-methyl butane freezes at about - 150 degrees C. I do not know if hexane has a lower freezing point but you can determine that with an internet search.
My point in all of this is that you may have other choices for snap freezing if you can't get an answer to your original question re hexane as long as you are able to freeze the needed temperature. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tracz...@aol.com Sent: Wednesday, August 12, 2009 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing in Hexane I have a procedure that refers to freezing a tissue sample in chilled Hexane. When I look in a chemical catalogue I find there are several formulas listed. Any direction on which one to use would be greatly appreciated. Thank you. Dorothy Dorothy Murphy Traczyk Murphy-Traczyk & Associates LLC PO Box 602 Point Pleasant, NJ 08742 _doro...@mtahistology.com_ (mailto:doro...@mtahistology.com) www.mtahistology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet