Peggy, Have you used this method of adhesion for special stains and IHC staining as well? If so, have you had any interference or background staining?
Thank you, Jackie Jacqueline D. Garfield | Manager, Histology Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1182 Email jgarfield@ lifecell.com www.lifcell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Thursday, August 20, 2009 8:03 AM To: Williams, Sean; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bone sections Marie, I make a 5% Titebond solution from Titebond II which can be purchased at any hardware store such as Home Depot, etc. and I rarely lose sections when H&E staining on paraffin embedded bones. Right before sectioning, I take a pipette using the 5% titebond and dab all the corners and center of the slide, take a kimwipe and spread it zigzag across one way all the way down the slide and then turn the slide 1/4 and do it again. Let it air dry a few seconds and then pick up your section. I should mention that I first clean the slides in acid alcohol for 5 minutes, followed by two 95% rinses for 5 minutes each and then dry in a 60 degree oven for 1 hour. Remove from oven, cool, wrap individually with either a kimwipe or paper towel to keep them protected from dust and store in an empty coverslip box. Hope this helps. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Williams, Sean Sent: Tuesday, August 18, 2009 07:51 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bone sections Hi Everyone Could any one tell me how they keep bone sections adhered to slides when staining. I'Ve tried polysine slides, pva glue slides, different ways of drying the sections and for different lenghts of time but nothing seems to work for every section. I'm only staining them with H&E. Thanks Marie O'Brien (liverpool vet path - histology) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet