Hi Jan:
We used to use glycerine+phenol for storing slices of embalmed cadavers
before embedding in clear plastic. I don't know the exact ratio but it
was mostly glycerin. We did not stain but painted the nerves, blood
vessels, etc as a teaching tool.
As for IHC, it would depend on the fixation and the antigen in question.
Geoff
Jan Shivers wrote:
I'm posting this for a colleague....
Does anyone have experience using glycerin/phenol as a storage medium, and if
so, for how long are the tissues still good for Histology processing/staining?
I assume they can't be used for IHC.
Thanks in advance,
Jan Shivers
University of Minnesota
Veterinary Diagnostic Laboratory
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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