I just purchased some from the VWR Catalogue, page 538, top left hand corner, disposable and reusable, 2009-2010 catalogue and you can access it online. Cheers,
Maureen Bukhari Phone: 403-210-6524 e-mail: mlbuk...@ucalgary.ca -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, August 07, 2009 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 69, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Core unit support (Va Paula Sicurello) 2. metal embedding base molds (Victoria Baker) 3. Support for core units (Va Paula Sicurello) 4. Re: metal embedding base molds (Amy Porter) 5. Mycoplasma bovis (Breeden, Sara) 6. RE: metal embedding base molds (Bernice Frederick) 7. Re: Mycoplasma bovis (jenee.s.od...@hawaii.gov) 8. (no subject) (Maria Katleba) 9. RE: metal embedding base molds (Nancy Klemme) 10. RE: metal embedding base molds (Jodie Robertson) 11. cement in bone specimen (DiCarlo, Margaret) 12. Re: Histonet Digest, Vol 69, Issue 6 (rick.garnh...@memorialhealthsystem.com) 13. What do you use for decolorizing after Hematox. (Michelle MacVeigh-Aloni) 14. RE: cement in bone specimen (Jack Ratliff) 15. Fetal demise placenta (mtitf...@aol.com) 16. HPGD, PAFa and frizzled-4 stainings on Ventana Benchmark (Vesterinen Tiina) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Aug 2009 10:12:31 -0700 (PDT) From: Va Paula Sicurello <vapat...@yahoo.com> Subject: [Histonet] Core unit support To: MSA BB <microsc...@microscopy.com>, HistoNet <histonet@lists.utsouthwestern.edu> Message-ID: <554043.34139...@web46112.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ------------------------------ Message: 2 Date: Thu, 6 Aug 2009 13:13:06 -0400 From: Victoria Baker <bakevicto...@gmail.com> Subject: [Histonet] metal embedding base molds To: histonet <histo...@pathology.swmed.edu> Message-ID: <4f016b690908061013r6a410546j8a867e9813392...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki ------------------------------ Message: 3 Date: Thu, 6 Aug 2009 10:14:19 -0700 (PDT) From: Va Paula Sicurello <vapat...@yahoo.com> Subject: [Histonet] Support for core units To: MSA BB <microsc...@microscopy.com>, HistoNet <histonet@lists.utsouthwestern.edu> Message-ID: <73199.21406...@web46104.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ------------------------------ Message: 4 Date: Thu, 6 Aug 2009 13:30:34 -0400 From: "Amy Porter" <port...@msu.edu> Subject: Re: [Histonet] metal embedding base molds To: "Victoria Baker" <bakevicto...@gmail.com>, "histonet" <histo...@pathology.swmed.edu> Message-ID: <08deaf2aef394d02817d86f01ad99...@histolab> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Try EM Sciences the distribute alot of the unique old type histology supplies. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Victoria Baker" <bakevicto...@gmail.com> To: "histonet" <histo...@pathology.swmed.edu> Sent: Thursday, August 06, 2009 1:13 PM Subject: [Histonet] metal embedding base molds > Hi- > > I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All > I'm > able to find at this point are the metal molds with the 'wings' on them. > > Does anyone know a source? With all the mergers etc it's been a little > challenging. > > Thanks in advance > > Vikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Thu, 6 Aug 2009 12:08:44 -0600 From: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Subject: [Histonet] Mycoplasma bovis To: <histonet@lists.utsouthwestern.edu> Message-ID: <4d14f0fc9316dd41972d5f03c070908b02e46...@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="US-ASCII" My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 6 Date: Thu, 6 Aug 2009 13:10:38 -0500 From: "Bernice Frederick" <b-freder...@northwestern.edu> Subject: RE: [Histonet] metal embedding base molds To: "'Amy Porter'" <port...@msu.edu>, "'Victoria Baker'" <bakevicto...@gmail.com>, "'histonet'" <histo...@pathology.swmed.edu> Message-ID: <b57f6f56230440f0aa69125a641ab...@lurie.northwestern.edu> Content-Type: text/plain; charset="us-ascii" As well as surgipath. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Thursday, August 06, 2009 12:31 PM To: Victoria Baker; histonet Subject: Re: [Histonet] metal embedding base molds Try EM Sciences the distribute alot of the unique old type histology supplies. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Victoria Baker" <bakevicto...@gmail.com> To: "histonet" <histo...@pathology.swmed.edu> Sent: Thursday, August 06, 2009 1:13 PM Subject: [Histonet] metal embedding base molds > Hi- > > I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All > I'm > able to find at this point are the metal molds with the 'wings' on them. > > Does anyone know a source? With all the mergers etc it's been a little > challenging. > > Thanks in advance > > Vikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 6 Aug 2009 08:19:25 -1000 From: jenee.s.od...@hawaii.gov Subject: Re: [Histonet] Mycoplasma bovis To: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Cc: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: <off539d4db.f9c49528-on0a25760a.00648e7d-0a25760a.00643...@hawaii.gov> Content-Type: text/plain; charset="US-ASCII" Hi there, Check out this database. It contains a contact list for the labs that are currently doing the Myco bovis IHC. http://ihc.sdstate.org/ -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 "Breeden, Sara" <sbree...@nmda.nmsu.edu> Sent by: histonet-boun...@lists.utsouthwestern.edu 08/06/2009 08:12 AM To <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Mycoplasma bovis My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 6 Aug 2009 11:23:21 -0700 From: Maria Katleba <maria.katl...@stjoe.org> Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <97c02552ecb11346877d3e83cf833abd13d17c4...@sjsnt-scmail03.stjoe.org> Content-Type: text/plain; charset="us-ascii" Hi All, Would anyone tell me what their lab procedure is for handling fetal demise placentas? In previous jobs sites, the histotech and/or PA would read the requisition and determine if there may be later DNA testing ordered. If yes, put in fridge and contact pathologist and/or physician. If not, place in formalin. Currently, our PA automatically places every placenta in formalin.... Kind regards, Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 9 Date: Thu, 6 Aug 2009 11:23:18 -0700 From: Nancy Klemme <nkle...@sakuraus.com> Subject: RE: [Histonet] metal embedding base molds To: Victoria Baker <bakevicto...@gmail.com>, histonet <histo...@pathology.swmed.edu> Message-ID: <782e3a02c2eb2347bea6dea69dc7ab865b4bff1...@sfamail.sakuraus.local> Content-Type: text/plain; charset="us-ascii" Dear Vikki, Sakura still carries the metal base molds that are used with Embedding Rings. They do not have the "wings" that the ones for use with the Unicassettes have. Their Sakura part numbers begin from 4121 and higher. If you use the electronic catalog on Sakura's website (www.sakuraus.com) and search by product description, just type in base molds. Then click on part # 4121 and the wingless ones will be pictured. Kind regards, Nancy Klemme, Sakura Educational Services -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 Aug 2009 12:24:53 -0700 From: "Jodie Robertson" <jrobert...@pathologysciences.com> Subject: RE: [Histonet] metal embedding base molds To: "Victoria Baker" <bakevicto...@gmail.com>, "histonet" <histo...@pathology.swmed.edu> Message-ID: <518cd6920aa7154193cbe5977cd880733a8...@psmgsrv2.psmg.local> Content-Type: text/plain; charset="us-ascii" Try Cardinal as they are a distributor for all Tissue Tek/Sakura supplies. Jodie Robertson, HT(ASCP)QIHC Histology Day Supervisor Pathology Sciences Medical Group -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 6 Aug 2009 15:43:16 -0400 From: "DiCarlo, Margaret" <mdica...@kaleidahealth.org> Subject: [Histonet] cement in bone specimen To: <histo...@pathology.swmed.edu> Message-ID: <1b73766a27a1554cb2729b6432e81301015c7...@kalexmb04.kaleidahealth.org> Content-Type: text/plain; charset="US-ASCII" Histonetters, We sliced a humerus bone on a band saw which was filled with cement from a previous surgery and my boss would like me to try to cut it on a sliding/sledge microtome. (The knife remains stationary) Is this possible to do? Presently, it is fixing and then I will decal it but I'm wondering if I'll be wasting my time and effort. I would appreciate anyone's experience with this. Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-7777. ------------------------------ Message: 12 Date: Thu, 6 Aug 2009 14:03:11 -0600 From: rick.garnh...@memorialhealthsystem.com Subject: [Histonet] Re: Histonet Digest, Vol 69, Issue 6 To: histonet@lists.utsouthwestern.edu Message-ID: <off5871773.d62f0e69-on8725760a.006ddfa9-8725760a.006e2...@memorialhealthsys tem.com> Content-Type: text/plain; charset=US-ASCII Does anyone out in histology have any documentation from CMS, ASCP, or NSH that breaks down the job duties or areas for what is covered under Tech and professional part of the CPT billing codes. For example is Grossing part of the a profee or tech part of the bill. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. ------------------------------ Message: 13 Date: Thu, 6 Aug 2009 13:03:33 -0700 From: "Michelle MacVeigh-Aloni" <macve...@usc.edu> Subject: [Histonet] What do you use for decolorizing after Hematox. To: <Histonet@lists.utsouthwestern.edu> Message-ID: <002401ca16d0$f9e4a3c0$5c237...@dfs66dd1> Content-Type: text/plain; charset="iso-8859-1" Hi all, In my H&E staining I always used 1% acid alcohol. (Working with conc. HCl is not fun!) There is the Orth's solution available already made, but it is 3%. Do you use that? How do you use it? What do you use in your regulat H&E stain? I am using it on an old Jung AUTOSTAINER. Michelle Research Specialist USC Keck School of Medicine ------------------------------ Message: 14 Date: Thu, 6 Aug 2009 16:21:41 -0400 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: RE: [Histonet] cement in bone specimen To: <mdica...@kaleidahealth.org>, <histo...@pathology.swmed.edu> Message-ID: <blu108-w219be87505ba8c878b7f82ae...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Peggy, If the cement you mention is or similar to PMMA, then you will not be able to cut thin sections using the sliding/sledge microtome. If I was you, I would not decal it at all as it will only make the bone less dense than the cement and will definitely waste your time if you embed it in paraffin to try and cut later. Your best and only chance at success here is to embed undemineralized in resin and cut thick or ground sections that can be polished to a desired thickness. If you would like to discuss this further, please give me a call at 317-281-1975. Best, Jack Ratliff > Date: Thu, 6 Aug 2009 15:43:16 -0400 > From: mdica...@kaleidahealth.org > To: histo...@pathology.swmed.edu > CC: > Subject: [Histonet] cement in bone specimen > > Histonetters, > > > > We sliced a humerus bone on a band saw which was filled with cement from > a previous surgery and my boss would like me to try to cut it on a > sliding/sledge microtome. (The knife remains stationary) Is this > possible to do? Presently, it is fixing and then I will decal it but > I'm wondering if I'll be wasting my time and effort. > > > > I would appreciate anyone's experience with this. > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > 2009 Best Places to Work Winner > Visit our careers page at www.kaleidahealth.org/careers > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 06 Aug 2009 16:53:16 -0400 From: mtitf...@aol.com Subject: [Histonet] Fetal demise placenta To: histo...@pathology.swmed.edu Message-ID: <8cbe4ef0e4c2479-93c-...@webmail-me19.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Maria asks about fetal demise placenta: We gross in all fetal demise placenta (usually four blocks).If the clinicians desire genetics, they take?samples from the fetus and the placenta up on the floor. (There is only a three day window for growing fibroblasts). The fetus gets a gross only treatment with weight, measurements and a photograph. If the clinicians or family desire an autopsy, whatever the gestational age, we have the parents sign an autopsy consent, as if for an adult. Regards Michael Titford USA Pathology Mobile AL ------------------------------ Message: 16 Date: Fri, 7 Aug 2009 09:05:35 +0300 From: Vesterinen Tiina <tiina.vesteri...@hus.fi> Subject: [Histonet] HPGD, PAFa and frizzled-4 stainings on Ventana Benchmark To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <2c0982d85c07a34598393f0b2b6f96754138868...@vanhusmbx06.hus.fi> Content-Type: text/plain; charset="iso-8859-1" Hi everyone, Has anyone stained formalin fixed, paraffin embedded slices with HPGD (Sigma), PAF acetylhydrolase (Cayman Chemical) or frizzled-4 (Santa Cruz) on Ventana Discovery, Benchmark or similar autostainer? I would appreciate all advices related to staining protocols because I4ve quite small amounts of antibodies. Regards, Tiina Vesterinen Tiina Vesterinen Project Researcher Institute for Molecular Medicine Finland FIMM Helsinki, Finland ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 69, Issue 7 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet