Anne, we do DIF on formalin fixed renal biopsies routinly. To get good results we perform Protease digestion before DIF. I don't have the protocol here, but we use Proteinase K from Dako at RT for 3-4 min, and the antibodies are between 1:20 and 1:40 für 30 min. I don't know, if that would help with glutaraldehyde-fixed specimen, but worth trying.
Gudrun Lang Akh Linz, Austria -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Anne van Binsbergen Gesendet: Donnerstag, 27. August 2009 12:24 An: John Kiernan; histonet@lists.utsouthwestern.edu Betreff: [Histonet] DIF tissue in GLUT Dear John and other knowledgeable Histonetters and Gurus of the trade.... Renal bx tissue core has been placed in Gluteraldehyde/Trumps EM fixative by mistake (we all make mistakes) - we now need to do DIF Does anyone out there have a method for this? -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet