Many thanks for the replies regarding this reagent. I delayed purchasing this as I was very skeptical: however, I can achieve a 1/5-10 fold dilution factor for my precious low affinity (? thus low dilution factor) Abs. I will not be using ImmPACT chromogen kit routinely as, for the vast majority of Abs, I do not find it neccessary. I have been informed ( courtesy of Histonetters who replied) that Dako offer a similarly sensitive kit. For the record, I routinely use a std manual stABCpx-DAB (DAB in-house prepared)detection system in a Research Lab with very little "clinical" time-constraints) carl
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