Hello,
I've tried a dozen times to get good fluorescent CD31 staining (endothelial
marker) on frozen mouse liver sections.
I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC
label, and get a very weak (though noticable) signal.
My question is regarding fixation methods IMMEDIATELY after dissection. Does
anyone have a good summary of fixation methods prior to freezing and their
effects on CD31 staining? Does the exact fixation even matter that much?
Everyone seems to have something different.
Thanks!
Jeff
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