Any decalcifying process, either acid or chelating, should always be conducted after the subject if fixed. René J.
--- On Fri, 9/25/09, Martina Sladkova <martina_sladk...@yahoo.com> wrote: From: Martina Sladkova <martina_sladk...@yahoo.com> Subject: [Histonet] Does EDTA destroy DNA during decalcifying process? To: histonet@lists.utsouthwestern.edu Date: Friday, September 25, 2009, 11:36 AM Hello ! Does anyone know whether EDTA destroys DNA during decalcifying process and whether it would be better to fix the sample before adding EDTA? My samples are natural coral cubes of 3mm. It is carbonate calcium. The coral cubes are seeded with mesenchymal stem cells. I need to extract DNA in order to evaluate cell proliferation. Thank you very much in advance, Martina SLADKOVA PhD student LABORATOIRE DE BIOINGENIERIE ET BIOMECANIQUE OSTEO-ARTICULAIRES (B2OA) CNRS UMR 7052 Faculté de médecine Lariboisière Saint-Louis Université Paris 7 10 avenue de Verdun 75010 PARIS Tél.: 0033 01 57 27 86 84 Fax : 0033 01 57 27 85 71 Email: martina_sladk...@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
