Under separate cover I sent an article for Stains for Plastic Embedded Tissue Sections which originally appeared in Stain Technology 42:9-14 (1974) by Humphrey and Pittman. I received it as an LKB applicaton note 303. The article describes an "H&E" stain for plastic sections, using methylene blue-azure II and basic fuchsin.
I used it a lot, with great success, when I worked at the Mass. Eye and Ear Infirmary. Peggy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Wednesday, September 30, 2009 11:12 AM To: histonet@lists.utsouthwestern.edu Cc: Pete Finger Subject: [Histonet] staining of plastic sections Hi Histonetters, If anyone has a good protocol for staining plastic sections, could they share it? We have samples embedded in an epoxy resin for TEM but they love the semi-thick sections on the glass slide and asked if we could do something other than a toluidine blue O, the standard stain for plastic. We have tried H&E and PAS with no success. If there some way to treat the sections in order to get them to stain? Is there another stain we could try? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet