Perhaps try freezing @ a higher temperature e.g C02. Regards Alan Bright Bright Instrument Co. Cambridgeshire UK Sent from my BlackBerry® wireless device
-----Original Message----- From: "Jean-Martin Lapointe" <jm.lapoi...@accellab.com> Date: Tue, 6 Oct 2009 10:08:03 To: <histonet@lists.utsouthwestern.edu> Subject: [Histonet] frozen myocardium sections Hi all, for a study we are freezing myocardium sections in OCT immersed directly in liquid nitrogen, without isopentane, because apparently isopentane quenches the fluorescence of the cells we need to detect in the tissue. Unfortunately the blocks tend to crack after freezing. Does anyone have a suggestion to avoid cracking ? thanks __________________________________ Jean-Martin Lapointe AccelLAB Inc jm.lapoi...@accellab.com <mailto:jm.lapoi...@accellab.com> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet