Depending on the tissue fixation & processing, you can see some cytoplasmic staining. How does the cell pellet control look? We seldom see cytoplasmic staining on that. Are you tracking that your retrieval buffer is 95-100 after you add the slides & starting your retrieval times when the buffer temp gets back up to 95? Wash well between all incubations, too. That's just some thoughts off the top of my head. Let me know if you have more specific questions on the protocol.
Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA > Does anyone have any experience using the Dako HerCep kit? > > And do you have any problems with cytoplasmic staining? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet