That will work but I'm not aware of the non-mouse anti-human macrophage marker, you could check the web or biocompare.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Margaryan, Naira [mailto:nmargar...@childrensmemorial.org] Sent: Friday, October 23, 2009 9:57 AM To: Liz Chlipala; Galbraith, Joe; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -----Original Message----- From: Liz Chlipala [mailto:l...@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbra...@uiowa.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet