Hi, Does anyone have any suggestions as to why our mouse glomeruli are shrinking after coverlipping in DPX? Immunohistochemistry is performed on PLP frozen cryostat sections, and the glomeruli and tubules look fine upon checking after the chromagen is developed, but once they are dehydrated, cleared in histosol, and then coverslipped in DPX(also tried with Vector hardmount) the glomeruli shrink, and the tubules look like they are spreading out. We have been doing this for years, and not had a problem. Does anyone know why we would have one now? Could the disease state of the tissue contribute? Or earlier fixation- could that contribute downstream?
Any suggestions would be appreciated- it is doing my head in! Kim O'Sullivan Department of Medicine Monash Univeristy Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet