Usually that is the result of incomplete fixation. Check your fixation protocol. René J.
--- On Mon, 1/11/10, Karen Cai <k...@prosci-inc.com> wrote: From: Karen Cai <k...@prosci-inc.com> Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax <http://www.prosci-inc.com> www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet