staining was performed on floating sections...and thus the incubation can reach the both sides of sections normally we use 12/24/48 well plate, add 1 ml/0.5ml/0.2ml antibody diluted solution and shake
widely used for brain section staining... the wash will be better, in the edge parts of sections 2010-01-15 TF 发件人： Karen Cai 发送时间： 2010-01-15 03:29:56 收件人： ti...@foxmail.com; 'Rene J Buesa'; 'histonet' 抄送： 主题： RE: Re: [Histonet] Eliminating the edge effect in IHC/IF Hi, Thank you very much for your input. Could you describe more with the floating section method? I never did it before. Best, Karen -----Original Message----- From: TF [mailto:ti...@foxmail.com] Sent: Wednesday, January 13, 2010 6:11 PM To: Rene J Buesa; histonet; Karen Cai Subject: Re: Re: [Histonet] Eliminating the edge effect in IHC/IF Hi all i believe it is due to the wash + antibody accumulation problem happened between the edge of section and the adhesive slide. Even you have circled the section with IHC pen(DAKO), and flattened all soultions evenly, it improved but did not disappear if you stain the section using "floating section" method, no edge effect now. 2010-01-14 TF 发件人： Rene J Buesa 发送时间： 2010-01-12 13:30:49 收件人： histonet; Karen Cai 抄送： 主题： Re: [Histonet] Eliminating the edge effect in IHC/IF Usually that is the result of incomplete fixation. Check your fixation protocol. René J. --- On Mon, 1/11/10, Karen Cai <k...@prosci-inc.com> wrote: From: Karen Cai <k...@prosci-inc.com> Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu Date: Monday, January 11, 2010, 2:00 PM Hi, I have a question for the generous input. When I do the IHC or IF, it seems very common that the intensity of the edge area of the tissue is always stronger than the central tissue part. Is it possible to eliminate this and make the staining evenly distributed around the whole tissue section? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax <http://www.prosci-inc.com> www.prosci-inc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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