For longer term preservation of brain sectins in floating state...0.01M PBS is not as good as 0.1 M PB..
2010-01-18 TF 发件人: Adam . 发送时间: 2010-01-18 11:02:26 收件人: tifei 抄送: Histonet@lists.utsouthwestern.edu 主题: Re: [Histonet] 0.1 M PB & 0.01M PBS I've used 1X PBS for everything and it seems to work. Adam On Sun, Jan 17, 2010 at 8:41 PM, TF <ti...@foxmail.com> wrote: We use 0.1 PB for tissue fixation (4% PFA), cryo-preservation (30% sucrose) then go 0.01 PBS for IHC though these are routines here, I wonder anyone can specify on the use of two different solutions, especially why? 2010-01-18 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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